The antiinflammatory activity of the plant extract was measured at a dose of 50 and 200 mgkg body weight against acute paw edema induced by carrageenan. Aquatic plant extracts used as soil amendments are most commonly derived from kelp, specifically 23 from ascophyllum spp. The activity drops down if the sample is freezethawed. Nov 26, 20 antioxidant composition and antioxidant activity. Analgesic activity of aqueous extract of vernonia hymenolepis. Preliminary phytochemical screening, quantitative estimation. New drugs from plant secondary metabolites are being developed and studied for their antibacterial and anticancer activities 5. In vivo and in vitro antidiabetic activity of terminalia. Evaluation of antidiabetic activity of plants used in western.
Susan srujana1, konduriraveendra babu1,bodavula samba siva rao2 1. Mahmood from the immunology laboratory of university of malaya. This study was based on screening antibacterial activity of the ethanol extract of baeckea frutescens l. Antidiabetic activity of vinca rosea extracts in alloxan. The main objective of this work is to evaluate the in vitro anti diabetic activity of methanolic extracts of cinnamomum zeylanicum, piper betle, artocarpus heterophyllus and artocarpus altilis. Trolox standard and plant working solutions a stock solution of a concentration of 1mgml in methanol was firstly prepared for the plant extract and trolox. Physalis minima is widely used in indian medicine by the tribal communities to treat.
American journal of research communication, 2015, 35. Further study to substantiate the use of the plant as antidiabetic is recommended. The stem bark of dialium guineense was collected and chopped into small pieces with a knife, dried under a mild sunlight and pulverised into a coarse powder of about 1mm in diameter. Fasting blood sugar level was lowered significantly by the plant extracts. Phytochemical analysis of methanolic extracts of leaves of. Increasingly, natural products have potential for the treatment of cancer. Antioxidant and in vitro cytotoxic activity of extracts of. Phytochemical and some pharmacological activity of acetone extracts of some. Aqueous extract of a particular plant was given to a particular experimental group for thirty days maintaining homogeneity in other variables to allow statistical analysis.
Preliminary phytochemical analysis of the extracts of. Polyherbal formulations, extracts and the bioactives have. Nematicidal activity of plant extracts against the rootknot nematode, meloidogyne incognita article pdf available in the open natural products journal 21 october 2009 with 2,728 reads. The working solutions of the following concentrations 1, 2, 3, 5, 7, 10, 20, 30, 40, 50, 80, 100 gml were prepared by serial. Mostly, this plant found europe and the northern usa forest 15,16. The leaves of clerodendrum viscosum possess flavonoids, alkaloids.
Phytochemical and some pharmacological activity of acetone extracts of some sudanese plants. The bilberry has several medicinal values for the treatment of various ailments including fever, cough, diabetic and liver disorder. The selected plant extracts were studied for their effect on inhibition of glycosylation of haemoglobin and glucose transport across yeast cells. Phytochemical and some pharmacological activity of acetone.
Antioxidant activities are known to increase proportionally tothe polyphenol content, mainly due to their redox properties. The present study was carried out to evaluate the antidiabetic activity of vinca rosea methanolic whole plant extracts in alloxan induced diabetic rats for 14 days. Cytotoxic and genotoxic effects of inula viscosa leaf. Phytochemical investigation and biological activity of leaves. The resulting extracts were subsequently weighted to produce 0. The methanolic whole plant extract at high dose 500 mgkg exhibited significant antihyperglycemic activity than whole plant extract at low dose 300 mgkg in diabetic rats. Antimicrobial activity of crude extracts of three medicinal. Depends on the target enzyme, pipette 1 ml reaction mixture into 1.
Plant extracts have a wide range of activities and their active secondary plant metabolites typically belong to the classes of isoprene derivatives and flavonoids tajodini et al. Browns college of pharmacy, khammam, andhra pradesh, india 2. Evaluation of the analgesic activity of the methanolic. Add 150 ul of the crude extract to the reaction mixture, vortex, and record the start point of time. Plant solutions were prepared by dissolving a known weight. Biological screening of various medicinal plant extracts. Evaluation of antidiabetic activity of plants used in.
Phytochemical investigation and biological activity of leaves extract of plant boswelliaserrata t. Dragendorffs test1 ml of extract, add 1 ml of dragendroffs reagent potassium bismuth iodidesolution. The findings from this study therefore support the folkloric usage of b. The prevalence of diabetes mellitus dm is increasing with ageing of the population and life style changes associated with rapid urbanization and westernization. The present study was designed to investigate the glucose uptake of antidiabetic activity crude nhexane,ethanol, methanol and aqueous leaf extracts of cardiospermum halicacabum. Phytochemical screening and antibacterial activity of.
Antidiabetic activity and phytochemical screening of. Jain5 1department of pharmacognosy, mcops, manipal576104, india. Aloe vera is a plant with turgid green leaves joined at the stem in a rosette pattern. In our study, the butanol fractions of selected plant species generally have been found more active than other fractions, indicating that compounds having antidiabetic activity are polar in nature which established the significant correlations of polyphenolic and flavonoids to lower down the diabetic effect. The extract was evaporated to get 10 ml of extract. In vitro study results scientifically supported the terminalia paniculata bark in vivo antidiabetic activity. Antidiabetic activity of terminalia catappa leaf extracts ijpt. Qualitative reactions for the detection of plant constituentstest for alkaloids1.
Cytotoxic studies of plant extracts on cancer cell lines zerumbone inhibited the proliferation of human colonic adenocarcinoma cell lines ls174t, ls 180, colo205 and colo320 dm in a dose dependent manner, while the growth of normal human dermal 2foc25 and colon ccd18 co fibroblasts was less affected. Ali 2010 tested the cytotoxic activity of flower decoction of hibiscus rosa sinensis using a. Antimicrobial activity of some plant extracts against. An inoculum suspension was swabbed uniformly to solidified 20 ml muellerhinton agar mha for bacteria and sabouraud dextrose agar sda for fungi and the. In vitro and in vivo antidiabetic effect of extracts of. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. It is acting as a source of plant growth hormones, carbohydrates, amino acids and vitamins. Open access toxicity and antifeedant activity of selected. Qualitative phytochemical screening tests were performed to detect phytochemicals in the extracts. A strong inhibition of the paw edema was observed with the different doses of the. Potential antidiabetic activity of extracts and isolated. The antimicrobial activity of the test organisms to the 11 plant extracts was screened by using the agarwell diffusion method perez et al.
Extracts from a broad spectrum of plant species contain substances that possess antitumor activity 1. The aim of the present study was to evaluate the in vitro cytotoxic activity of methanolic and aqueous extract of aerial parts of cocculus hirsutus on mcf7 breast cell lines. In vitro antidiabetic activity of cardiospermum halicacabum leaves extracts by stalin. This observed antidiabetic activity of this extract might be due to their phytochemical constituents previously reported. Evaluation of antidiabetic activity of medicinal plant. The dose of each extract was calculated according to body weight before administration to the diabetic rats. The extract was cooled and excess of sodium sulphate was added.
The amount of the plant extract dosage given to each rat was initially based on the calculated animals body weight. The dose dependent effects of 28 days oral treatment with methanol extract 100, 200 and 400 mgkg from the plant of m. It is found in many consumer products including beverages, skin lotion, cosmetics, or ointments for minor burns and sunburns perkins, 2016. Antibacterial activity of leaf extracts of baeckea. To the extract 25 ml of 10% naoh was added, then it was boiled in water bath for 30 min. The antidiabetic activity in plants may be attributed to the presence of phenolic and flavonoid compounds. Extraction and determination of antioxidant activity of. Antimicrobial activity of crude extracts of some plant leaves. Antinociceptive and antiinflammatory properties of daucus. Eucalyptus camaldulensis, phytochemical screening, aqueous extract, ethanolic extract. The extraction was done by cold maceration method in 80% methanol for 48hrs with intermittent shaking at 2 hrs interval.
The extract was filtered using what man filter paper no. An orangered precipitate indicates the presence of alkaloids. In the stzinduced diabetic mice, both extracts showed a strong antihyperglycemic activity, with the group receiving 50 mgkg of ethanol extract and the group receiving 50 mgkg of aqueous extract presenting 64. Mayers test1 ml of extract, add 1 ml of mayers reagent potassium mercuric. Assessment of antioxidant activity of plant extracts by. In vitro antidiabetic activity and mechanism of action of. Radical scavenging activity of plant extract was measured by method of brandwilliams et al. Phytochemical studies and biological activity of dodonaea. It is also observed by sugumaran and vetrichelvan 2008 that tannins are absent in ethyl acetate extract of bouhinia purpurea linn. Modern extraction methods for preparation of bioactive plant extracts. Evaluation of the analgesic activity of the methanolic stem.
Aquatic plant extracts agricultural marketing service. Plant leaves are rich in flavonols and other pigments. Pdf nematicidal activity of plant extracts against the. The phytochemical constituents of eucalyptus camaldulensis plant sections aqueousextracts are presented in table 1, while. A comparison was made between the action of costus igneus extract and a known antidiabetic drug glibenclamide 600 gkg body wt.
Evaluation of in vitro anti diabetic activity of selected. Antisnake venom activity of different extracts of pouzolzia indica against russel viper venom anas ahmed1, k. Hypoglycemic and hypolipidaemic effects of some common. Preliminary phytochemical analysis of the extracts of psidium. Further, aqueous extract of terminalia paniculata bark contains active biomarkers which may possibly be responsible for the antidiabetic activity of terminalia paniculata bark. In vitro study on antiglycation activity, issn antioxidant. In this study we evaluated the leaf crude extracts of g. Qualitative and quantitative phytochemical analysis of. It was extracted up to one week with daily 2 hours stirring with a mechanical stirrer. Moreover, antimicrobial activity of different natural substances such as medicinal plant extract have been investigated against food borne bacteria. In this study the prolonged effect up to 14 day of the methanolic extracts of whole plant of vinca rosea in fasting blood glucose fbg and biochemical parameters such as serum total cholesterol tc, ldl, hdl, creatinine, urea, and alkaline phosphatase were studied in alloxan induced diabetic rats. Hence, the chemical constituents of the plant extract might help in preventing diabetic complications and may serve as an alternative in the present armamentarium of antidiabetic drugs.
Extraction of plant contituents linkedin slideshare. However, due to the potential toxicity of the plant extract it must be prescribed with caution. Numerous studies have been carried out to extract various natural products for screening antimicrobial activity 15 but attention has not been focused intensively on studying the combinations of these products for their antimicrobial activity. Antibacterial activities of some plant extracts utilized. A strong inhibition of the paw edema was observed with the different doses of the extract and with diclofenac standard drug. Alkaloids, flavonoids, terpenoids, carbohydrates, protein and amino acids were analysed. Department of pharmacy, khammam college of pharmacy, khammam, andhra pradesh, india. The scientific articles evidenced that the fruit has antidiabetic and hypoglycemic activity 17. Ethanol extract six grams of the powdered plant material was extracted in a soxhlet extractor using 25ml of 40% ethanol. Nematicidal activity of plant extracts against the rootknot nematode, meloidogyne incognita article pdf available in the open natural products.
Plant extract properties plant extracts consists mainly of proteins, peptides, oligosaccharides, fatty acids, vitamins, micro minerals. For treatments, the concentration of aqueous extract was 400 mgkg body weight. The parenchyma cells contain a transparent mucilaginous jelly. Vegetative growth, chemical composition, and flavonoids. The present study deals with the analysis of phytochemical constituents by qualitative and quantitative analysis of moringa concanensis leaves, flowers and seeds were done using methanol extract. Ba and ca plants containhigher polyphenol levels than the other plants tested table 1. Small quantity of extract was pressed the between two filter papers, the stain on ist filter paper indicated the presence of fixed oils. Plants and plant products have been used extensively throughout history to treat medical problems. Antiinflammatory and analgesic activity of methanolic. The aim of this research was to investigate antidiabetic activity of costus igneus also known as insulin plant leaves extracts in alloxan induced diabetic albino rats. Gut3 1department of plant protection, agriculture faculty, gaziosmanpa. This was prepared by dissolving 2 g of plant extract in 25 ml water in a falcon tube in a hot water bath with vigorous shaking.
The plant caused reduction in the duration of time spent in pain behavior for aqueous extract in both the early phase 56. Rajendaran1, durga jaiswal2, hari pratap singh3, ashutosh mishra3, dinesh chandra4, indranil kumar yadav5and d. Antioxidant activity in plant oil plant oils contain unsaturated fatty acids, which are most sensitive to oxidative degradation. Due to these reasons, the aim of this paper was to investigate the antioxidant and antimicrobial activity of the plum seed extract, as well as the antiproliferative activity of isolated amygdalin.
After 7 days the extract was filtered through muslin cloth and marc was discarded. Extraction and determination of antioxidant activity of withania somnifera dunal abdul qaiyum ansari 1, syed abrar ahmed1, m. Blood samples were collected at the end of the experiment and biochemical parameters were analyzed. Pdf nematicidal activity of plant extracts against the root. Antibacterial activity of leaf extracts of baeckea frutescens.
Phytochemical investigation and biological activity of. High antioxidant activity was seen with acetone extracts of a. In this activity, the students will be testing the effectiveness of several types of plant extract antibiotics, which they have produced from plants selected from their local ecosystem. Potential antidiabetic activity of medicinal plants a. The water extract of osbeckia octandra significantly lowered the fasting glucose level and markedly improved glucose tolerance in spraguedawley rats and the maximum hypoglycemic activity was observed after 3 hours 7. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31.904 20 1188 1280 1564 1222 1159 1440 198 1307 1040 420 736 780 1518 913 322 107 444 29 867 450 847 737 1248 515 956 985 1460 1175 1038 113 1036 435 1035